Table 1. List of primer sequences used for qPCR analysis

Gene	Forward primer (5′ → 3′)	Reverse primer (5′ → 3′)
TYMS	CCAAAGCTCAGGATTCTTCG	AGTTGGATGCGGATTGTACC
TYMP	CAATGATCAGCGGACGTGG	ACTCTGACCCACGATACAGC
TK1	CACAGAGTTGATGAGACGCG	CTGTAGCGAGTGTCTTTGGC
MTHFR	CTTTGAGGCTGACCTGAAGC	AAGCGGAAGAATGTGTCAGC
CCND1	TGCTGGTTTTCTACCCAACG	AGTGCTTGGAAATGGAATGG
CCNE1	GGGGAGCTCAAAACTGAAGC	ACATGGCTTTCTTTGCTCGG
CCNA2	AGCACTCTACACAGTCACGG	GGTAGGTCTGGTGAAGGTCC
FOXM1	GGTACCTATCCAGTTCCCGG	TCTGAGCTCATGAGGGAAGC
CHEK1	TGTAGATATGAAGCGTGCCG	TTGCCTTCTCTCCTGTGACC
MCM4	GTTCACCACTGACATACGGC	CAGACTGCAGATCCACTTGC
GAPDH	TCGTGGAAGGACTCATGACC	ATGATGTTCTGGAGAGCCCC

qPCR, quantitative real-time PCR; TYMS, thymidylate synthase; TYMP, thymidine phosphorylase; TK1, thymidine kinase 1; MTHFR, methylenetetrahydrofolate reductase; CCND1, cyclin D1; CCNE1, cyclin E1; CCNA2, cyclin A2; FOXM1, forkhead box M1; CHEK1, checkpoint kinase 1; MCM4, minichromosome maintenance protein 4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.